Xenogen ivis 100 system software

Xenogen, living image, ivis, dlit and kinetic are protocol 11. This unit has a maintenance schedule showing it was last serviced in 820. Feb 19, 2015 this training video discusses using the ivis platform for in vivo bioluminescence imaging studies to evaluate 2 separate mouse tumor models. For in vivo imaging, mice were injected intraperitoneally with 4. Living image advanced in vivo imaging software designed for the ivis lumina platform simplifies even the most complex image acquisition and analysis of 2d bioluminescent and fluorescent probes. The system uses leading optical imaging technology to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Enhanced dermal wound neovascularization by targeted delivery of endothelial. The ivis spectrum is equipped wit h a strong bottom illumination fluorescence light source. For our experiment well use the ivis spectrum in vivo imaging system uses a backthinned charge coupled device cooled to 90c to achieve maximum sensitivity.

Please click on examples of research to view images obtained at umms. We utilized in vivo imaging systems ivis in conjunction with a firefly bioluminescent enzyme to progressively track the invasion of the central nervous system cns by an encephalitic virus in a murine model. To acquire an image on the ivis lumina, ivis spectrum. The ivis spectrum advanced preclinical optical imaging system combines high throughput and full tomographic optical imaging in one platform. The cmir xenogen ivis imaging system 100 consists of a backilluminated ccd with a closedcycle refrigeration system, a camera controller, and a lighttight box for bioluminescence imaging. Ivis is a wine production, vineyard management, and quality management system qms. Integrated into the system are both a bioluminescence system and a fluorescence system and the capability to easily switch between modalities.

If it is necessary to relocate the ivis lumina, contact xenogen technical support. Initialize the ivis system and confirm or wait for the ccd temperature to lock. Ivis system comparison ivis lumina lt ivis lumina iii ivis lumina xrms ivis lumina s5 ivis lumina x5 ivis spectrumbl ivis spectrum ivis spectrumct animal capacity 3 mice 5 mice 10 mice 5 mice 10 mice with manifold upgrade 5 mice 10 mice with manifold upgrade 2d bioluminescence chemiluminescence v v v v v v v v cerenkov. Csu surplus property is selling an ivis 100 imaging system previously used in a university setting. Instrumentation small animal imaging facility core. Utilizing luciferase and in vivo imaging systems ivis as a novel means to identify disease endpoints before clinical developments occur. Living image software 3d analysis package was designed for the ivis imaging system 3d, xenogen s latest advance in imaging technology, and to be compatible with the companys ivis imaging system 200 to provide more spatially accurate and quantitative data than the current software. Sep 11, 2015 xenogen ivis 100 imaging system for auction. To start imaging, initialize the ivis system one click and set the imaging parameters for the experiment. Route of delivery influences biodistribution of human bone. The system uses leading optical technology for preclinical imaging research and development ideal for noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. We offer ivis in two sizes to accommodate both small and large animals, each of which can be used to analyze multiple animals simultaneously in a.

In living image software, exposure time, fstop and pixel binning can be optimized based on the expression level of. Ivis consists of a highlysensitive camera, an ultradark box that serves as the imaging chamber and a computer equipped with our livingimage software. The xenogen ivis system combines the ability to image in vitro or in vivo, fluorescence or bioluminescence, and 2d or 3d. The xenogen ivis 100 system from caliper life sciences may be used for both bioluminescence and fluorescence. The status light is green when the system is ready for operation. Jul 14, 2009 an anesthesia system is hooked to the stage for isoflurane delivery to animals. We have used the ivis 200 imaging system for over 2 years now. The ivis lumina is sensitive, scientific equipment and should not be moved by any user unless the system is located on the xws260 workstation. An anesthesia system is hooked to the stage for isoflurane delivery to animals. The ivis spectrum in vivo imaging system combines 2d optical and 3d optical tomography in one platform. Xenogen ivis 100 in vivo imaging system university. Accelerating research with ivis lumina s5 and x5 highthroughput systems. In vivo bioluminescent imaging of mammary tumors using ivis.

In vivo imaging systems ivis detection of a neuroinvasive. The xenogen ivis 100 is a noninvasive, realtime system forin vivoimaging of bioluminescence and fluorescence. The ivis lumina s5 2d optical imaging system combines high sensitivity optical and patented spectral unmixing capabilities in a highthroughput system. Xenogen ivis 100 in vivo imaging system standard operation. Pixel density was measured using unscanit gel analysis software version 5. Tissues were preserved in rnalater qiagen, valencia, ca and.

Ivis fluorescent imaging quick start guide ivis fluorescent imaging quick start guide 1 acquiring an image first take an image of your subject to determine the best settings for your images. Silica and titanium dioxide nanoparticles cause pregnancy. Nov 12, 2014 for sale ivis 100 ccd camera xenogen imaging tech system with cryotiger compressor. To support absolute quantitation, the system measures dark charge during downtime and runs a selfcalibration during initialization. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. The ivis 100 system and living image software both from xenogen, inc. Sustained high level transgene expression in mammalian. Xenogens ivis 200 series and living image software. Luminescence images with 1 sec exposure and photographic images were overlaid using xenogen living image software perkin elmer. Ivis technologies transforming the way organizations. The ivis spectrum is a bioluminescence and fluorescence in vivo imaging system, which allows for noninvasive tracking and measurement of disease progression, cellular activity and gene expression. All imaging components are contained within a portable cabinet occupying a. We found that green fluorescent proteinexpressing cells 100 x 10 6 were not detectable in a mouse cadaver phantom experiment. For sale ivis 100 ccd camera xenogen imaging tech system with cryotiger compressor.

Xenogen corporation announces record 2005 revenue up 28%. Advanced acquisition and analysis tools for ivis lumina imaging systems. Sustained, high level transgene expression in mammalian cells is desired in many cases for studying gene functions. Ivis imaging system 3d, 200, 100, and 50 series models living image software for digital image processing and analysis on windows and macintosh computer platforms light producing cells and microorganisms, including bacteria and human tumor cells tagged to emit light. Small animal imaging l equipment and scheduling city of. In this context, bioluminescence results from enzymemediated chemical reactions involving injected substrates. Bioluminescence data were analysed using living image version 4. After 24 h, optical images of the whole body, maternal liver and placenta were acquired with a xenogen ivis 200 imaging system. Using this staggered system, large sets of animals can be processed in a relatively short period of time. E weight data was recorded from the excised tumors at sacrifice day 21.

After isoflurane inhalation anesthesia, the animals were injected intraperitoneally ip with 100. Integrated into the system are both a bioluminescence system and a fluorescence system and the capability to. Located within the university of chicagos carlson animal research facility, the optical imaging core facility oicf provides quantitative fluorescence and bioluminescence imaging services for in vivo and in vitro studies of small animals and specimens using three different modalities. Luciferase expression and bioluminescence does not affect. The faciliys imaging instruments include the xenogen ivis 200 imaging system, the olympus ov100 in vivo. The living image software automatically coregisters the images to generate an overlay image figure 2. May 16, 2005 living image software 3d analysis package was designed for the ivis imaging system 3d, xenogen s latest advance in imaging technology, and to be compatible with the companys ivis imaging system 200 to provide more spatially accurate and quantitative data than the current software. Ivis technologies transforming the way organizations engage. Enhanced dermal wound neovascularization by targeted. Transduction, tropism, and biodistribution of aav vectors. In the living image software, click initialize ivis system in the ivis system control panel figure 7. Nov 22, 2010 after 21 days, bioluminescent imaging was performed on tumors expressing gfpp2aluc c using the xenogen ivis 100. Living lmage software version 2 university of chicago.

Ivis spectrum 4 multimodality coregistration fluorescence ct coregistration analysis the ivis spectrum is the most advanced in vivo imaging system available on the market today not only can it quantitate and localize 3d fluorescent and bioluminescent sources in vivo, but it can import and automatically coregister. Xenogen releases 3d software for its proprietary imaging systems. Images from the above three systems may be sent to any user by dicom or through the web by ipacs software. D images were analyzed using living image software and represented as total flux measurements in photonssecond. Mice are normally under anesthesia during imaging to prevent movement, with heat provided to maintain body temperature. The growth in ivis system revenues was lead by over 100 % growth in unit sales of our ivis 200 systems and increased average pricing for all ivis systems versus 2004.

The system includes a highly sensitive, cooled, backthinned ccd, a light tight imaging. It relies on patented optical imaging technologies to facilitate noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. Xenogen free culture medium for stem cells inventors igor slukvin, gene uenishi since its founding in 1925 as the patenting and licensing organization for the university of wisconsinmadison, warf has been working with business and industry to transform university research into products that benefit society. Ivis spectrum in vivo imaging system university imaging.

The field of view can be adjusted from 1025 cm to allow the imaging of two to. Xenogen ivis 50 bioluminescence imaging system is an eaglei resource of type in vivo bioluminescence imaging system at harvard university. Perkinelmer has led the way for over 25 years with powerful software and informatics solutions. In vivo bioluminescent imaging of mammary tumors using. In vivo bioluminescent imaging of mammary tumors using ivis spectrum.

An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to. Superhighmagnetization nanocarrier as a doxorubicin. The ivis spectrum is the most versatile and advanced in vivo imaging system available on the market today. The system provides researchers with greater insights into complex biological systems by enabling simultaneous molecular and anatomical noninvasive imaging in animal models. Noninvasive optical tracking of red fluorescent protein. Xenogen ivis 100 in vivo imaging system university imaging. Data were analyzed with living image software xenogen. The ivis spectumct preclinical in vivo imaging system expands upon the versatility of the ivis spectrum by offering 2d and 3d imaging capabilities but includes integrated lowdose microct ideal for longitudinal studies. Luminescence was detected using a xenogen ivis lumina imaging system caliper life sciences, hopkinton, ma. Xenogen ivis200 central microscopy research facility. Combinatorial targeting of cancer bone metastasis using. Because we do not have all the pieces necessary to fully test this unit, it has not been tested, the overall condition of the unit is unknown and latent defects may exist. Silica and titanium dioxide nanoparticles cause pregnancy complications in mice. If the file is in a zip format double click the zip file and choose unzip and select a location on your computer to save the files.

For those of you who have previously used our ivis 100, you will find that this new system is truly state of the art in comparison. Ivis spectrum in vivo imaging system university imaging centers. All imaging components are contained within a portable cabinet occupying a 25x34 inch footprint. Xenogen releases 3d software for its proprietary imaging. Typically, the disease progresses relatively slowly, however virus replication is rapid, especially within the cns, and can lead to an. With streamlined interfaces and workflows for longitudinal analysis of 2d data including coregistration with xray in applicable ivis lumina systems, living image in vivo imaging software sets the industry standard for.

Initialize system by clicking the initialize ivis system button in. The soundlimiting enclosure beneath the imaging chamber contains the. The ivis controls dashboard gives you a quick overview of all core elements you have installed in your instance. The xenogen ivis200 system is a highsensitivity, low noise, in vivo imaging technology platform that allows noninvasive visualization and tracking of cellular and genetic activity within a living organism, in real time. Started in 2002 and supported by some of the best winemakers in the u. The ivis imaging system 200 series is calipers most advanced imaging system, combining the ability to image in vitro or in vivo, fluorescence or bioluminescence, and 2d or 3d, in a single integrated imaging system.

We successfully launched our ivis 3d system in 2005 with three initial sales, which could build nicely going forward, said david w. Xenogen ivis 100 in vivo imaging system standard operation protocol basic operation for acquiring image 1. Living image software is a custom software package developed by xenogen that runs the ivis system and provides tools for image display and analysis. Noninvasive bioluminescence imaging in small animals.

The ivis imaging system is a modu lar software and hardware system. Due to the risk of potential damage, it is critical that only a trained technician moves the ivis lumina. Ivis has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. In vivo images were collected with a fluorescence imaging system xenogen ivis 100, caliper life sciences, hopkinton, ma. Tissue autofluorescence was subtracted by acquiring separate images obtained from a specific gfp background excitation. We have evaluated the use of the xenogen ivis 200 imaging system for realtime fluorescence proteinbased optical imaging of metastatic progression in live animals. After in vivo bli at the last time point, mice were injected with an additional dose of 200. Computer is kept always on, no need to turn on computer 3. The xenogen ivis 200 system is a highsensitivity, low noise, in vivo imaging technology platform that allows noninvasive visualization and tracking of cellular and genetic activity within a living organism, in real time. Right now we have only cybersecurity and we can quickly see that we have 24 completed, 15 controls with a poam, 17 not applicable controls, and 54 incomplete controls. In vivo bioluminescence imaging on the ivis platform youtube. Mutation of the fiber shaft heparan sulphate binding site.

With streamlined interfaces and workflows for longitudinal analysis of 2d data including coregistration with xray in. Visible light with a central wavelength at 649 nm was used as the excitation source. Living image software can be fully utilized with the new ivis imaging system 200 series and is compatible with existing ivis imaging system 100 series and ivis imaging system 50 series technology. The ivis 100 imaging system is a highly sensitive and large field of view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. Wait until the camera has cooled to operating temperature requires about ten minutes. Ivis fluorescent imaging guide university of chicago. Xenogen ivis 50 bioluminescence imaging system at harvard. Selling ivis 100 ccd camera xenogen imaging tech system on. Use the links below to quickly find your software solution download. Superhighmagnetization nanocarrier as a doxorubicin delivery platform for magnetic targeting therapy. Whether your concerns are compliance, planning, work control, cost tracking, or quality management, ivis handles it. Its capable of imaging bioluminescence and fluorescence. This program is designed for either those organizations that have already built a complete nist cybersecurity plan and are leveraging the ivis compliance platform or those who need recommendations on how to complete their plan to put into the ivis compliance platform.

The xenogen ivis imaging system is a highly sensitive, low lightlevel system optimized for in vivo whole, living animals imaging. The gene vector transduction was monitored by luminescence evaluated in vivo with an imaging system xenogen ivis. In the ivis system control panel that appears, do the following. The living image software supports hardware for ivis imaging system 50, 100, 200, 3d series and lumina see table 1.

To acquire an image on the ivis lumina, ivis spectrum, ivis imaging system 100 series or 200 series. The system provides researchers with greater insights into complex biological systems by enabling simultaneous molecular. Software and mouse imaging shuttle injection system, anesthesia, calibration tools, phantom mice, multimodality software. The ccd is linked to a computer where images can be retrieved and analyzed using the proprietary living image software. In this drug study, tumor signals vary by three orders of. Sign on log book once you entered the animal imaging room. Right click on the link to the file and choose save target as and save the zip file to your desktop. Welcome 2 the ivis spectrum is an integrated imaging system that includes a. Software images are acquired in a two step process living image controls ivis and provides image analysis tools fluorescence attention must be paid to auto fluorescence issues filter selection and lamp are controlled by living image xenogen. Ivis innovative vinology information system ivis software. After a 15 min hold time peak of enzyme kinetics, tumour bioluminescence data were collected after 1 s and 1 min integration times using a xenogen ivis lumina ii multispectral imager caliper lifesciences, waltham, ma, usa.